Angiotensin-1 and vimentin expression and ultrastructural examination in severe preeclampsia complicated by HELLP syndrome changes in the structure of the umbilical cord
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Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Verduci Publisher
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
OBJECTIVE: The purpose of this study was to examine the histopathologic, ultra -structural and immunohistochemical changes in the umbilical cord in women diagnosed with HELLP syndrome.MATERIALS AND METHODS: Postpartum umbilical cords of 40 patients at the 35-38th week of pregnancy were included. 20 severe preeclamptic (HELLP) and 20 normal umbilical cords were used. After the follow-up of tissue parts of 10% formaldehyde solution for histopa-thology and immunohistochemistry, histopatho-logical and angiopoietin-1 and vimentin antibod-ies were examined as immunohistochemical af-ter routine paraffin follow-up. For electron mi- croscope analysis, umbilical cord samples were taken into 2.5% glutaral aldehyde solution.RESULTS: In the statistical comparison, mean difference in increased diameter and addition-al anomaly on the ultrasound of preeclamptic patients was statistically different compared to control patients. In the HELLP group, hyperpla-sia and degenerative changes, pyknosis of the endothelial cell nuclei of the vessels and apop-totic changes in some regions were observed. Immunohistochemical analysis showed that en-dothelial cells, basal membrane and fibroblast cells in the HELLP group expressed high levels of vimentin. Angiotensin-1 expression was in-creased in amniotic epithelial cells, endothelial cells and some pericyte cells.CONCLUSIONS: As a result, it was observed that the signaling that started with trophoblastic invasion with the effect of hypoxia in severe pre-eclampsia and continued with dysfunction in en-dothelial cells was parallel to the increase in an-giotensin and vimentin receptors. It is thought that the ultrastructural change in endothelial cells may cause disruption of the collagenized structure in Wharton gel, which supports this, and may cause adverse effects in fetal develop-ment and nutrition.
Açıklama
Anahtar Kelimeler
Hellp Syndrome, Angiotensin-1, Vimentin, Umbil-Ical Cord
Kaynak
European Review For Medical and Pharmacological Sciences
WoS Q Değeri
N/A
Scopus Q Değeri
Q2
Cilt
27
Sayı
6
