Di-, and triterpenoids isolation and LC-MS analysis of salvia marashica extracts with bioactivity studies
Yükleniyor...
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
ACG Publications
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Abstract: In this study, dichloromethane, acetone, and methanol extracts of the aerial parts of the Salvia
marashica plant which is an endemic species to Anatolia, were investigated. The total phenolic amounts of these
extracts were determined as pyrocatechol equivalent and total flavonoids as quercetin equivalent. Antioxidant
activity was determined by four complementary methods including inhibition of lipid peroxidation (by βcarotene color expression), DPPH free radical scavenging activity, ABTS cation radical scavenging activity and
CUPRAC methods. Anticholinesterase activity of the extracts was investigated by the Ellman method against
acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. Viability and cytotoxic activity tests
were carried out on the fibroblast L929 cells and cytotoxic A549 lung cancer cells, respectively. The
triterpenoids and diterpenoids constitute the major secondary metabolites of the S. marashica acetone and
methanol extracts isolated by chromatographic methods. Their structures were determined based on
spectroscopic methods, namely NMR and mass analyses. Ten terpenoids were obtained from either acetone or
methanol extracts of the S. marashica. Seven of them were triterpenoids, elucidated as lupeol, lupeol-3-acetate,
lup-12, 20(29)-diene, lup-20(29)-ene, α-amyrin-tetracosanoate, oleanolic acid and ursolic acid besides a steroid
β-sitosterol. Two abietane diterpenes, abieta-8,11,13-triene (1) and 18-acetoxymethylene-abieta-8,11,13-triene
(2), were obtained from the acetone extract which were isolated from a Salvia species for the first time in the
present study. The methanol extract was found to be very rich in rosmarinic acid determined by LC-MS/MS
analysis.
Açıklama
WOS:000658306500004
Anahtar Kelimeler
Salvia marashica, Terpenoids, Flavonoids, NMR, LC-MS/MS, Bioactivity
Kaynak
Records of Natural Products
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
15
Sayı
6
Künye
Aydın, S.K., Ertaş, A., Boğa, M., Erol, E., Toraman, G.O.A., Saygı, T.K. ve diğerleri. (2021). Di-, and triterpenoids isolation and LC-MS analysis of salvia marashica extracts with bioactivity studies. Records of Natural Products, 15(6), 463-475.
