Chromosomal abnormalities in non-obstructive azoospermic men prior to employment of assisted reproduction in Southeast Turkey
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Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Japan International Cultural Exchange Foundation
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objective: The causes of male infertility are heterogeneous but more than 50% of cases have a genetic defect. Chromosomal abnormalities that affect on gametogenesis are one of the principle genetic factors in male infertility. The aim of this study is to determine the frequency and type of chromosomal abnormalities in non-obstructive azoospermic men with severe male factor infertility to give appropriate genetic counseling before assisted reproduction techniques. Materials and Methods: A total of 114 azoospermic infertile males were studied for the cytogenetic evaluation prior to use of assisted reproduction techniques. A detailed history was taken for each man. Karyotyping was performed on peripheral blood lymphocytes according to standard methods. Results: The overall incidence of chromosomal abnormalities was about 22.8% (26/114), including the sex chromosome abnormality 19.3% and the autosomal chromosome abnormality 3.5%. Twenty one of 22 patients with sex chromosome abnormality had classic Klinefelter karyotype. There were 2 mosaic cases involving X and Y chromosomes. Of the four cases with autosomal chromosome anomalies, three cases had balanced reciprocal translocations and one case with inversion. FSH, LH and testosterone levels showed significant increase in azoospermic patients with abnormal karyotype when compared with the normal karyotype (P < 0.05). Conclusions: Our findings are generally in accordance with those from other surveys and confirm that the XXY aneuploidy is the most frequent chromosomal abnormality in azoospermic individuals. The occurrence of chromosomal anomalies among infertile males suggests the need for genetic screening and proper genetic counselling before initiation of assisted reproduction treatment. © 2015 Japan Health Sciences University.
Açıklama
Anahtar Kelimeler
Assisted Reproduction Techniques, Azoospermia, Chromosome, Karyotype Analysis, Male İnfertility
Kaynak
International Medical Journal
WoS Q Değeri
Scopus Q Değeri
N/A
Cilt
22
Sayı
4
