Production, purification, and characterization of thermostable ?-amylase from thermophilic Geobacillus stearothermophilus

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dc.contributor.authorFincan, Sema Agüloğlu
dc.contributor.authorEnez, Barış
dc.date.accessioned2024-04-24T15:59:33Z
dc.date.available2024-04-24T15:59:33Z
dc.date.issued2014
dc.departmentDicle Üniversitesi, Fen Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractThe -amylase (-1-4-D-glucan glucanohydrolase; EC 3.2.1.1) secreted by Geobacillus stearotermophilus was purified and characterized. Maximum enzyme production was achieved after 24h cultivation at pH 7.0 and 55 degrees C. The enzyme was active in a broad temperature range, between 50 and 80 degrees C, with an optimum at 70 degrees C; and maximum activity was at pH 7.0. The enzyme was purified using 80% ammonium sulfate precipitation, dialysis, Sephadex G-100 gel filtration, and DEAE-cellulose column chromatography, with a 46-fold and 65% recovery and showed a MW of 63kDa by SDS-PAGE. It was determined that the purified enzyme was stable at 50 and 60 degrees C, and pH 7.0. It was determined that the purified enzyme was stable at 50 and 60 degrees C at the end of 2h. The enzyme retained 100% activity pH 7.0 at the end of 3h. The enzyme was activated by Ca2+, Mn2+, and Triton X-100, but strongly inhibited by Cu2+, Zn2+, Fe2+, and Hg2+. The enzyme follows Michaelis-Menten kinetics with K-m and V-max values of 0.051mM and 1.424mol/min, respectively.en_US
dc.identifier.citationFincan, S.A. ve Enez, B. (2014). Production, purification, and characterization of thermostable a-amylase from thermophilic Geobacillus stearothermophilus. Starch/Staerke, 66(1-2), 182–189.
dc.identifier.doi10.1002/star.201200279
dc.identifier.endpage189en_US
dc.identifier.issn0038-9056
dc.identifier.issn1521-379X
dc.identifier.issue1-2en_US
dc.identifier.scopus2-s2.0-84895061353
dc.identifier.scopusqualityQ2
dc.identifier.startpage182en_US
dc.identifier.urihttps://doi.org/10.1002/star.201200279
dc.identifier.urihttps://hdl.handle.net/11468/14136
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/epdf/10.1002/star.201200279
dc.identifier.volume66en_US
dc.identifier.wosWOS:000330592000018
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorFincan, Sema Agüloğlu
dc.institutionauthorEnez, Barış
dc.language.isoenen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofStarch-Starke
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlpha-amylaseen_US
dc.subjectGeobacillus stearothermophilusen_US
dc.subjectProductionen_US
dc.subjectPurificationen_US
dc.titleProduction, purification, and characterization of thermostable ?-amylase from thermophilic Geobacillus stearothermophilusen_US
dc.titleProduction, purification, and characterization of thermostable ?-amylase from thermophilic Geobacillus stearothermophilus
dc.typeArticleen_US

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