Chemical Compositions by Using LC-MS/MS and GC-MS and Biological Activities of Sedum sediforme (Jacq.) Pau
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Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Amer Chemical Soc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this research, the chemical composition and biological activities of various extracts obtained from whole parts of Sedum sediforme (Jacq.) Pau were compared. The amounts of total phenolic and flavonoid components in crude extracts were determined by expression as pyrocatechol and quercetin equivalents, respectively. All of the extracts (petroleum ether, acetone, methanol, and water) obtained from S. sediforme showed strong antioxidant activity in four tested methods. Particularly, the IC50 values of the methanol extract, which was the richest in terms of total phenolic and flavonoid contents, were found to be lower than those of alpha-tocopherol and BHT in beta-carotene bleaching (9.78 +/- 0.06 mu g/mL), DPPH free radical scavenging (9.07 +/- 0.07 mu g/mL), and ABTS cation radical scavenging (5.87 +/- 0.03 mu g/mL) methods. Furthermore, the methanol extract of S. sediforme showed higher inhibition activity than galanthamine against acetyl- and butyryl-cholinesterase enzymes. Also, acetone and methanol extracts exhibited moderate antimicrobial activity against Candida albicans. The main constituents of fatty acid and essential oil were identified as palmitic acid (C16:0) (28.8%) and alpha-selinene (20.4%), respectively, by GC-MS. In the methanol extract of S. sediforme, quercetin, rutin, naringenin, and protocatechuic, p-coumaric, caffeic, and chlorogenic acids were detected and quantified by LC-MS/MS. Results of the current study showed that the methanol extract of S. sediforme may also be used as a food supplement.
Açıklama
Anahtar Kelimeler
Sedum Sediforme, Phenolic Content, Essential Oil, Fatty Acid, Antioxidant, Anticholinesterase, Antimicrobial, Quercetin, Lc-Ms/Ms, Gc-Ms
Kaynak
Journal of Agricultural and Food Chemistry
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
62
Sayı
20
