The role of prenatal steroid and TRH treatment in rats with nitrofen induced diaphragmatic hernia
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Tarih
2000
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The mortality of congenital diaphragmatic hernia (CDH) is high due to associated pulmonary hypoplasia and pulmonary hypertension. Thus studies aiming to prevent pulmonary hypoplasia in the prenatal period are gaining importance. In this study the role of glucocorticoids and "Thyroid releasing hormone" (TRH) on lung maturation in rats with nitrofen induced CDH is investigated. Ten pregnant Wistar-Albino female rats received nitrofen (100 mg) in two groups on their 9.5 day of pregnancy, while the control group had olive oil administered. In one nitrofen group additionally starting on day 16, glucocorticoids (0, 25 mg/kg betametazon) and TRH (25 ?g/kg) was administered for 5 days. Fetuses were delivered by ceaserean section on the 21st day of gestation and the fetal lungs were histopathologically investigated and DNA analysis and maturation were obtained. Nine fetuses in the control, 14 in the nitrofen and 7 in the treatment group could be investigated. No significant difference was identified in the morphologic evaluation and DNA analysis of the nitrofen and treatment groups. Histopathology revealed hypoplasia that was more prominent in the left lung in both groups. In conclusion the prenatal administration of TRH and glucocorticoids in prevention of nitrofen induced CDH has not been found effective. We believe this may be related to the initiation of treatment on day 16 of gestation.
Açıklama
Anahtar Kelimeler
Congenital Diaphragmatic Hernia, Fetal Disease, Fetal Organ Maturity, Fetal Therapy, Pulmonary Hypertension, Steroids, Thyroid Hormones
Kaynak
Pediatrik Cerrahi Dergisi
WoS Q Değeri
Scopus Q Değeri
N/A
Cilt
14
Sayı
2
