Effect of multiple low-dose PGF2? injections on the mature corpus luteum in non-pregnant bitches

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dc.contributor.authorUcar, Eyyup H.
dc.contributor.authorCetin, Hayrettin
dc.contributor.authorAtli, Mehmet O.
dc.date.accessioned2024-04-24T16:18:29Z
dc.date.available2024-04-24T16:18:29Z
dc.date.issued2018
dc.departmentDicle Üniversitesien_US
dc.description.abstractThis study investigated molecular regulation in the canine corpus luteums/corpora lutea (CL) following multiple low-dose prostaglandin F-2 alpha (PGF(2)alpha) injections in non-pregnant bitches around 30-35 days after ovulation. The CL were obtained by ovariohysterectomy 1 h after the last PGF(2)alpha injection. The subjects were divided into the following groups: control (no PGF(2)alpha injection, n = 4), one PGF(2)alpha injection (injection at 0 h, 1PGF, n = 4), two PGF(2)alpha injection (injection at 0 and 8 h, 2PGF, n = 4), and three PGF(2)alpha injection (injection at 0, 8 and 24 h, 3PGF, n = 4). In the 1PGF group, the steady-state mRNA levels of an immediate early gene (NR4A1) and immune system-related genes (MCP-1 and IL-8) increased. NR4A1 was localized in luteal and endothelial cells. In contrast, MCP-1 was localized in the luteal tissue between the luteal and endothelial cells. LHCGR, CYP11A1, and StAR mRNA expression decreased after the second PGF(2)alpha injection. FASLG increased only after the third PGF(2)alpha injection. The mRNA levels of PTGFR, PGT, and PTGS2 decreased as the number of PGF(2)alpha injections increased. Immunohistochemistry showed a decrease in StAR protein density as the number of PGF(2)alpha injections increased. BAX and CASP3 mRNA expression levels were similar among the groups. Serum progesterone (P-4) levels decreased dramatically after the PGF(2)alpha injections but were still higher than the basal level at the end of the study. In conclusion, repeated low-dose PGF(2)alpha injections could induce luteolytic mechanisms in the CL of non-pregnant bitches. Furthermore, it can be concluded that, in non-pregnant bitches, some aspects of the molecular regulation of luteolysis in the CL are similar to some aspects of such regulation in other domestic animals. (C) 2018 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipAdnan Menderes University Scientific Research Foundation [VTF-15013]; Dicle University Scientific Research Foundation (DUBAP) [14-VF-112]en_US
dc.description.sponsorshipThis study was supported by Adnan Menderes University Scientific Research Foundation (VTF-15013) and Dicle University Scientific Research Foundation (DUBAP) (14-VF-112 to Mehmet O. Atli). The authors would like to thank Drs Mehmet KOSE and M. Salih KAYA for their helps in laboratory procedures.en_US
dc.identifier.doi10.1016/j.theriogenology.2018.01.018
dc.identifier.endpage43en_US
dc.identifier.issn0093-691X
dc.identifier.issn1879-3231
dc.identifier.pmid29454296
dc.identifier.scopus2-s2.0-85042054239
dc.identifier.scopusqualityQ1
dc.identifier.startpage34en_US
dc.identifier.urihttps://doi.org/10.1016/j.theriogenology.2018.01.018
dc.identifier.urihttps://hdl.handle.net/11468/16127
dc.identifier.volume113en_US
dc.identifier.wosWOS:000431091900005
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofTheriogenology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectProstaglandinf2 Alphaen_US
dc.subjectLutelysisen_US
dc.subjectGene Expressionsen_US
dc.subjectCorpus Luteumen_US
dc.subjectBitchesen_US
dc.titleEffect of multiple low-dose PGF2? injections on the mature corpus luteum in non-pregnant bitchesen_US
dc.titleEffect of multiple low-dose PGF2? injections on the mature corpus luteum in non-pregnant bitches
dc.typeArticleen_US

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