Screening of Various Organic Substrates and the Development of a Suitable Low-Cost Fermentation Medium for the Production of ?-Amylase by Bacillus subtilis
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Tarih
2009
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Faculty Food Technology Biotechnology
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The production of extracellular amylase by Bacillus subtilis has been studied in solid-state fermentation (SSF). In a sequential order, various process parameters were optimized for maximum amylase production. The tested process parameters were different solid substrates such as banana husk (BH), water melon husk (WMH), lentil bran (LB), wheat bran (WB), melon husk (MH) and maize oil cake (MOC), different incubation time (24-144 h), particle size (500-2500 mu m), initial moisture content of the substrate (40-70 %, by mass per Volume), inoclum size (10-60 %, by mass per volume) and inoculum concentration (10-60 % by volume per mass). The maximum production of amylase (4857 U/mg) was achieved when 1 % starch (by mass, particle size of 1500 pm) was added to banana husk as the solid substrate, fermented for 72 h at 37 degrees C, at an inoculum level of 30 % (by volume per mass), and initial moisture content of 60 % (by volume per mass)
Açıklama
Anahtar Kelimeler
Bacillus Subtilis, Alpha-Amylase, Banana Husk, Solid-State Fermentation
Kaynak
Food Technology and Biotechnology
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
47
Sayı
4
