Investigation of Chemical Composition and Biological Activity of Salix aegyptiaca L. Roots
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Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Kahramanmaras Sutcu Imam Univ Rektorlugu
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The root of Salix aegyptiaca L. was extracted using their yield percentage, total condensed tannin concentration, antimicrobial, antioxidant activity and to determine chemical composition by LC-MS/MS. The root extraction was carried out together with water, ethanol and methanol. Accelerated solvent extraction (ASE), conventional extraction (CE), and microwave extraction (ME) were the extraction methods applied during the investigation. The LC-MS/MS methanol extract was used to detect phenolics. The antioxidant activities and total condensed tannin concentrations of root extracts have been done by UV-visible spectroscopy from 517 to 580 nm, severally. The disk diffusion method was used for antimicrobial activity. The maximum extraction yield (17.2%) was obtained in methanol by the ASE technique whereas, the conventional extraction technique obtained the minimum extraction efficiency (9.1%). By triplicate measurement, the total condensed tannin analysis result was found 35.14 mg/L. Using the ASE technique, the methanol extract was the maximum inhibitory zone (26 mm) against Candida albicans ATCC 10231. However, in water extract by conventional extraction, a minimum inhibitory zone (11 mm) was obtained against Staphylococcus aureus Cowan 1. The highest and lowest DPPH scavenging activity was determined in methanol (ASE) (98.8%) and ethanol (97.5%) extract respectively. The maximum amounts of quinic acid (63895 mu g/g) were discovered using LC-MS/MS.
Açıklama
Anahtar Kelimeler
Antioxidants, Antimicrobial, Chemical composition, Condensed tannin, Salix aegyptiaca
Kaynak
Ksu Tarim Ve Doga Dergisi-Ksu Journal of Agriculture and Nature
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
27
Sayı
6
