Adsorption of cellulase on poly(2-hydroxyethyl methacrylate) cryogels containing phenylalanine
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Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Tubitak Scientific & Technological Research Council Turkey
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The aim of this study was to prepare a supermacroporous cryogel that can be used for the adsorption of cellulase. The macroporous cryogel of poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-phenylalanine) [p(HEMA-MAPA)] was prepared by copolymerization of 2-hydroxyethyl methacrylate (HEMA) with a functional monomer of N-methacryloyl-L-phenylalanine (MAPA). The cryogel was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and swelling tests. The effects of several parameters such as medium pH, temperature, ionic strength, and flow rate on cellulase adsorption were also investigated. Maximum cellulase adsorption was observed at 25 degrees C and pH 4.0. Furthermore, adsorbed cellulase was desorbed from the cryogel by using 1.0 M NaCl. The p(HEMA-MAPA) cryogel could be used many times without the cellulase adsorption capacity decreasing significantly.
Açıklama
Anahtar Kelimeler
Cellulase, Adsorption, Cryogel, P(Hema-Mapa)
Kaynak
Turkish Journal of Chemistry
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
40
Sayı
5
