The use of Bacillus subtilis immobilized on Amberlite XAD-4 as a new biosorbent in trace metal determination
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Tarih
2007
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Bv
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The present work proposes the use of Bacillus subtilis immobilized on Amberlite XAD-4 as new biosorbent in trace metal determination. The procedure is based on the biosorption of Cu and Cd ions on a column of Amberlite XAD-4 resin loaded with dried, dead bacterial components prior to their determination by flame AAS. Various parameters such as pH, amount of adsorbent, eluent type and volume, flow rate of solution and matrix interference effect on the retention of the metal ions have been studied. The optimum pH values of quantitative sorption for Cu and Cd were found to be 7.0 and 7.5, respectively. These metal ions can be desorbed with 1 M HCl (recovery, 96-100%). The,sorption capacity of the resin was 0.0297 and 0.035 mmol g(-1) for Cu(2+) and Cd(2+), respectively. The tolerance limit of some electrolytes were also studied. This procedure was applied to Cu(2+) and Cd(2+) determination in aqueous solutions, including river and well water systems. In order to evaluate the accuracy of the proposed procedure, the certified reference materials, NRCC-SLRS-4 Riverine water and LGC7162 Strawberry leaves were analyzed. (C) 2007 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
Bacillus Subtilis, Preconcentration, Amberlite Xad-4, Trace Metal, Atomic Absorption Spectrometry
Kaynak
Journal of Hazardous Materials
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
149
Sayı
1
