Comparative study of the quantitative resolution of paracetamol and methocarbamol mixture by spectrophotometry with wavelet transform and UPLC techniques
Yükleniyor...
Tarih
2022
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The aim of this study was to develop a new simple and cost-effective spectrophotometric method to be used as an alternative to chromatography for the analysis of paracetamol and methocarbamol (PAR-MET) combination. In this regard, a comparative study on the quantitative resolution of PAR and MET mixture using continuous wavelet transform (CWT) and ultra-performance liquid chromatography (UPLC) techniques was performed. In interpretation of the spectrophotometric data, three different wavelet families Biorthogonal3.5 (Bior3.5), Mexican Hat Function (Mexh), and Symlets5 (Sym5) were found suitable to get better transform of zero-order spectra of the samples containing PAR and MET. The concentrations of PAR and MET in the working range of 4.0 - 20.0 mu g/mL and 5.0 - 45.0 mu g/mL, respectively, were proportional to the wavelet amplitudes. This mathematical relationship was used to construct calibration curves for each compound. After validation of the CWT method, analyses of commercial tablets were successfully performed. A new UPLC method was developed and validated in order to compare the results with those obtained by the CWT method and the data were found comparable. The analysis of results obtained from experimental trials showed that the proposed CWTmethod is suitable for the quality control and quantitative assessment of both active substances in tablet formulations.
Açıklama
Anahtar Kelimeler
Spectrophotometry, Wavelet transform, Ultra-performance liquid chromatography, Paracetamol, Methocarbamol
Kaynak
Pharmaceutical Chemistry Journal
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
55
Sayı
10
Künye
Güzel, R., Ertekin, Z.C., Ziyadanoğulları, B., Dinç, E. ve Ziyadanoğulları, R. (2022). Comparative study of the quantitative resolution of paracetamol and methocarbamol mixture by spectrophotometry with wavelet transform and UPLC techniques. Pharmaceutical Chemistry Journal, Pharmaceutical Chemistry Journal, 55(10), 1126-1132.
