Akut myeloid lösemili hastalarımızda t(8;21) ve inv (16)'nın kantitatif RT-PCR ile araştırılması
Yükleniyor...
Tarih
2005
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Akademi Doktorlar Yayınevi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Akut myeloid lösemilerde (AML) kromozomal anomaliteler ile prognoz aras›nda ilişki olduğu gösterilmiştir. Çok
say›da farkl› kromozomal anomaliteler aras›nda t(8;21) ve inv(16)'n›n varl›ğ›n›n iyi prognoz göstergesi olduğu kabul
edilmektedir. Diğer kromozomal anomaliteler ise kötü prognostik değer olarak kabul edilmekte ve tedavi plan› bunlara göre düzenlenmektedir. Biz de bu amaçla kliniğimizde tan› konulan 19 AML hastas›nda kantitatif reverz transkripsiyon polimeraz zincir reaksiyonu (RT-PCR) metoduyla t(8;21) ve inv(16) anormalliklerini inceledik. Ayr›ca tüm
hastalara klasik sitogenetik inceleme yap›ld›. Hastalar›n 9'u erkek,10'u bayand›. Yaş aral›ğ› 19-58’idi. Morfolojik
olarak 5'i AML-M1, 8'i AML-M2, 5'i AML-M4, 1'i AML-M5 tipinde idi. Hastalar›n biri 2.indüksiyonda olmak üzere
tüm hastalar ilk indüksiyon tedavisiyle remisyona girdi. Sitogenetik incelemede bir hastada t(8;21) saptand› ve RTPCR metoduyla t(8;21) teyid edildi. Diğer hastalarda yap›sal ve say›sal olarak herhangi bir kromozomal düzensizliğe
rastlanmad›.
Bu çal›şmay› sunmam›zdaki amaç; AML'li olgular›m›zda t(8;21) ve inv(16) anomalitelerini göstermektir.
It has been showed that there is a relation between chromosomal abnormality and prognosis in acute myeloid leukemia (AML). There are many chromosomal abnormalities in AML patients and it is known that t(8;21) and inv(16) are good prognostic abnormalities. Recently the treatment has been planned according to these abnormalities. We investigated t(8,21) and inv(16) abnormalities by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) in 19 patients with AML. Morever conventional cytogenetic method was applied for all patients. Of whom 9 were men and 10 women. Age of avarage was 19 to 58 years. FAB morphologic classification follows as; 5 AML-Ml, 8 AML-M2, 5 AML-M4, 1 AML-M5. Complete remission was obtained in eighteen patients with first induction regimen, whereas in one patient after second induction. t(8;21) has been found in 1 patient with cytogenetic method and confirmed with RT-PCR. In this study, we aim to search t(8;21) and inv (16) abnormalities in AML cases.
It has been showed that there is a relation between chromosomal abnormality and prognosis in acute myeloid leukemia (AML). There are many chromosomal abnormalities in AML patients and it is known that t(8;21) and inv(16) are good prognostic abnormalities. Recently the treatment has been planned according to these abnormalities. We investigated t(8,21) and inv(16) abnormalities by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) in 19 patients with AML. Morever conventional cytogenetic method was applied for all patients. Of whom 9 were men and 10 women. Age of avarage was 19 to 58 years. FAB morphologic classification follows as; 5 AML-Ml, 8 AML-M2, 5 AML-M4, 1 AML-M5. Complete remission was obtained in eighteen patients with first induction regimen, whereas in one patient after second induction. t(8;21) has been found in 1 patient with cytogenetic method and confirmed with RT-PCR. In this study, we aim to search t(8;21) and inv (16) abnormalities in AML cases.
Açıklama
Anahtar Kelimeler
Akut myeloid lösemi, t(8;21), inv(16), Kantitatif reverz transkripsiyon polimeraz zincir reaksiyonu, Acute myeloid leukemia, Quantitative reverse transcription polymerase chain reaction
Kaynak
Türk Hematoloji Onkoloji Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
15
Sayı
1
Künye
Ayyıldız, O., Kalkanlı, S., Batun, S., Işıkdoğan, A., Söker, M., Yurt, M. ve diğerleri. (2005). Akut myeloid lösemili hastalarımızda t(8;21) ve inv (16)'nın kantitatif RT-PCR ile araştırılması. Türk Hematoloji Onkoloji Dergisi, 15(1), 15-17.
