The contribution of nitric oxide on the relaxation effects of diethylstilbestrol
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Tarih
2008
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Saudi Med J
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Objectives: To evaluate the contribution of nitric oxide (NO) on the relaxation effects of diethylstilbestrol on rat uterus. Methods: Uterine rings from 8 nonpregnant Wistar Albino rats (300-350g) in the pro-estrous phase were suspended in an organ bath and electrical field stimulation applied for recording isometric tension. The influence of NO on contractile responses of rat uterine rings was investigated. The effects of NO precursor L-arginine (10(-7)-10(-4)M) concentration and NO synthase inhibitor L-nitro-arginine-methyl ester (10(-7)-10(-4)M) concentration and a combination of them on contractile responses were studied in the presence and absence of diethylstilbestrol (2x10(-4)M) concentration. The study was carried out at the Department of Pharmacology Laboratory, Faculty of Medicine, Dicle University, Diyarbakir, Turkey. Results: Totally, 30 samples were investigated (n=6 for each group, 5 groups). Diethylstilbestrol inhibited contractile responses 64.2 +/- 4.5% (n=6, p<0.05). Contractile responses decreased in the presence of Larginine (n=6, p<0.05) and this inhibition was abolished in the presence of L-nitro-arginine-methyl ester (n=6, p<0.05). The inhibition on contractile responses to diethylstilbestrol was potentiated in the presence of Larginine under similar conditions (n=6, p<0.05). The contractile responses to electrical field stimulation in the presence of diethylstilbestrol were not affected by L-nitro-arginine-methyl ester (n=6, p>0.05). Conclusions: These data provide evidence that NO may potentiate the inhibitory effects of diethylstilbestrol by different mechanisms on the electrically induced contractions of the non-pregnant rat uterus.
Açıklama
Anahtar Kelimeler
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Kaynak
Saudi Medical Journal
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
29
Sayı
5
