Effects of gallic acid on ischemia-reperfusion induced testicular injury in a rat testicular torsion model
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Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
SCI Printers & Publication INC
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
OBJECTIVE: Testicular torsion is a common urological complication mostly affecting adolescents and young men. In this study we investigated biochemical and immunohistochemical effects of gallic acid on the damage induced by testicular torsion-detorsion.
STUDY DESIGN: Forty male rats were divided into 4 groups of 10 animals each: control, torsion, torsion/detorsion, and torsion/detorsion + gallic acid. Testicles were removed from the scrotum, and 2.5-hour ischemia was applied to the left testis by twisting the spermatic cord 720 degrees clockwise. Then 3-hour reperfusion was allowed for detorsion. Gallic acid 20 mg/kg was orally administered to the torsion/detorsion + gallic acid group before reperfusion. Biochemical parameters of testicular tissue (MDA, SOD, CAT, and GSH levels) were measured. Testicular tissues were immune-stained with caspase-3 and TNF-alpha antibody.
RESULTS: MDA levels in the torsion/detorsion + gallic acid group were close to those in the control group; however, it was higher in the torsion/detorsion group as compared to the control group. Compared to control group, SOD, CAT, and GSH activities were significantly increased in the torsion/detorsion + gallic acid group. However, those values were decreased in the torsion-detorsion group. Spermatogenic cells and interstitial cells showed positive caspase-3 expression in the torsion and torsion-detorsion groups; however, expression level was decreased in the torsion/detorsion + gallic acid group. TNF-alpha expression was observed in degenerated spermatogonia, Leydig cells, and macrophages in the torsion and torsion-detorsion groups. In the torsion/detorsion + gallic acid group, TNF-alpha expression was observed in some interstitial region rather than in cells of seminiferous tubules.
CONCLUSION: Gallic acid treatment could be an alternative therapy in testicular ischemia-reperfusion injury to decrease inflammation, germ cell degeneration, and apoptosis.
Açıklama
WOS:000640456900001
Anahtar Kelimeler
Detorsion, Ischemia-reperfusion injury, Oxidative stress, Rats, Wistar, Reperfusion injury, Spermatic cord torsion, Spermatogenesis, Testicular torsion, Testis, Torsion abnormality, Torsion-detorsion
Kaynak
Analytical and Quantitave Cytopathology and Histopathology
WoS Q Değeri
N/A
Scopus Q Değeri
N/A
Cilt
43
Sayı
1
Künye
Söğütçü, N. ve Yokuş, B. (2021). Effects of gallic acid on ischemia-reperfusion induced testicular injury in a rat testicular torsion model. Analytical and Quantitave Cytopathology and Histopathology, 43(1), 1-7.
