THE PREPARATION OF A NEW PROTEIN BASED HPLC COLUMN PACKING MATERIAL AND ENANTIOMERIC RESOLUTION OF SOME PHARMACEUTICAL RELATED COMPOUNDS VIA THIS COLUMN
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Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Bilal GÜMÜŞ
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, BSA was bounded to 3-chloropropyl functionalized silica gel via ethylenediamine and glutaric dialdehyde. The prepared CSP was packed into 150X4.6mm HPLC column according to slurry method. The enantiomeric resolution of some pharmaceutical related compounds such as rac-mandelic acid, rac-methyl mandelate, rac-epinephrine, rac-propranolol, rac-?-phenylethylamine and rac-3-hydroxybutyric acid was employed in 1mL/rpm, 1,5mL/rpm, 2mL/rpm, 2,5mL/rpm, 3mL/rpm flow rate in various pHs such pH=6, pH=7, pH=8 0,1M phosphate buffer. Chromatographic parameters such as Rs and ? were calculated for each resoluted compounds.
Açıklama
Anahtar Kelimeler
Enantiomeric separation, hplc, bovine serum albumin
Kaynak
Middle East Journal of Science
WoS Q Değeri
Scopus Q Değeri
Cilt
2
Sayı
1
