PCR and ELISA for staphylococcal enterotoxins and detection of some exotoxins from Staphylococcus spp. strains by PCR

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dc.contributor.authorKahya, Serpil
dc.contributor.authorGuran, Husnu Sahan
dc.contributor.authorYilmaz, Ozge
dc.date.accessioned2024-04-24T17:37:44Z
dc.date.available2024-04-24T17:37:44Z
dc.date.issued2016
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe aims of this study were to determine the existence of some staphylococcal enterotoxin (SE) (sea, seb, sec, sed, and see) proteins and genes in coagulase-positive staphylococci (CPS) by ELISA and PCR and to assess SE-like toxin (SEI) (seg, seh, sei, sej, sem, sen, and seo), exfoliative toxin (eta and etb), toxic shock syndrome toxin-1 (tst) and 16S rRNA genes in 11 different Staphylococcus strains [90 CPS and 118 coagulase-negative staphylococci (CNS)] isolated from 250 ground meat samples by either monoplex or multiplex PCR. SEs were identified in 36 (40%) out of 90 CPS isolates by both ELISA and PCR, with the following distribution: sea was identified in 7 (7.7%), seb in 5 (5.5%), sec in 3 (3.3%), sed in 4 (4.4%), and see in 17 (18.8%). In addition, a total of 90 CPS and 118 CNS isolates were investigated for the presence of 11 SE, SEI, eta-etb, tst, and 16S rRNA genes. Overall, 145 (69.7%) of the Staphylococcus spp. isolates tested positive for one or more toxin genes. These results indicate that CNS may play an important role in food poisoning and that SEI toxins must be investigated in greater detail in future studies of both CPS and CNS.en_US
dc.identifier.endpage33en_US
dc.identifier.issn0025-8628
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84968735783
dc.identifier.scopusqualityQ4
dc.identifier.startpage28en_US
dc.identifier.urihttps://hdl.handle.net/11468/21152
dc.identifier.volume72en_US
dc.identifier.wosWOS:000367758700006
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherPolish Soc Veterinary Sciences Editorial Officeen_US
dc.relation.ispartofMedycyna Weterynaryjna-Veterinary Medicine-Science and Practice
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectStaphylococcus Spp.en_US
dc.subjectEnterotoxinsen_US
dc.subjectExotoxinsen_US
dc.subjectElisaen_US
dc.subjectPcren_US
dc.titlePCR and ELISA for staphylococcal enterotoxins and detection of some exotoxins from Staphylococcus spp. strains by PCRen_US
dc.titlePCR and ELISA for staphylococcal enterotoxins and detection of some exotoxins from Staphylococcus spp. strains by PCR
dc.typeArticleen_US

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