Purification of glucose 6-phosphate dehydrogenase from chicken erythrocytes. Investigation of some kinetic properties

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dc.contributor.authorYilmaz, H
dc.contributor.authorCiftci, M
dc.contributor.authorBeydemir, S
dc.contributor.authorBakan, E
dc.date.accessioned2024-04-24T17:07:54Z
dc.date.available2024-04-24T17:07:54Z
dc.date.issued2002
dc.departmentDicle Üniversitesien_US
dc.description.abstractGlucose 6-phosphate dehydrogenase (G6PD) was purified from chicken erythrocytes, and some characteristics of the enzyme were investigated. The purification procedure was composed of three steps: hemolysate preparation, ammonium sulfate precipitation, and 2',5'-ADP Sepharose 4B affinity gel chromatography. Thanks to the three consecutive procedures, the enzyme, having the specific activity of 20.862 EU/mg proteins, was purified with a yield of 54.68% and 9,150-fold. Optimal pH, stable pH, optimal temperature, molecular weight, and K-M and V-max values for NADP(+) and glucose 6- phosphate (G6-P) were also determined for the enzyme. In addition, K-i values and the type of inhibition were determined by means of Line-Weaver-Burk graphs obtained for such inhibitors as ATP, ADP, NADH, and NADPH.en_US
dc.identifier.doi10.1080/PB-120013475
dc.identifier.endpage301en_US
dc.identifier.issn1082-6068
dc.identifier.issn1532-2297
dc.identifier.issue3en_US
dc.identifier.pmid12375816
dc.identifier.scopus2-s2.0-0036403737
dc.identifier.scopusqualityQ2
dc.identifier.startpage287en_US
dc.identifier.urihttps://doi.org/10.1080/PB-120013475
dc.identifier.urihttps://hdl.handle.net/11468/17088
dc.identifier.volume32en_US
dc.identifier.wosWOS:000178535000006
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofPreparative Biochemistry & Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject[No Keyword]en_US
dc.titlePurification of glucose 6-phosphate dehydrogenase from chicken erythrocytes. Investigation of some kinetic propertiesen_US
dc.titlePurification of glucose 6-phosphate dehydrogenase from chicken erythrocytes. Investigation of some kinetic properties
dc.typeArticleen_US

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