Suitability of animals' purified milk caseins and their subunit ?-caseins as substrates for subtilisin and trypsin
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Tarih
2001
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Marcel Dekker Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Acid casein and K-casein were purified from different species of animal's milk, such as cow, sheep, goat, and water buffalo. These caseins were used as substrates for commercially available subtilisin and trypsin. It was established that, when acid caseins were used as a substrate for subtilisin, cow acid casein was found to be a better substrate for the enzymes, compared to other animals' milk casein. It was suggested that this acid casein has significantly more aromatic amino acids, as compared to arginine and lysine. K-m and V-max values, which were obtained for cow K-casein, showed that cow K-casein was a better susbstrate for trypsin than the others, suggesting that cow Ic-casein has a rich content of lysine, arginine, and aromatic amino acids by comparison with the others. The calculated C/N ratio also supports this suggestion.
Açıklama
Anahtar Kelimeler
[No Keyword]
Kaynak
Preparative Biochemistry & Biotechnology
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
31
Sayı
2
