Anti-Cataract effect of the traditional aqueouse extract of yerba mate (Ilex paraguariensis A. St.-Hil.): An in ovo perspective

Özet

Introduction: The therapeutic effect of different doses of the traditional aqueous extract of dried leaves of yerba mate (Ilex paraguariensis A. St.-Hil.) was investigated in an experimental cataract model in chicken embryos. Methods and Results: LC-MS/MS analysis allowed the identification and quantification of 53 metabolites. In the hydrocortisone-induced cataract model, lenses were examined morphologically after treatment and parameters related to oxidative stress (total antioxidant/oxidant status (TAS/TOS), glutathione (GSH), and malondialdehyde (MDA)) were evaluated. Antiproliferative cell nuclear antigen (PCNA) and caspase-3 H-scores were determined and crystallin alpha A (CRYAA) gene expression in the lenses was measured by RT-PCR. The degree of cataract decreased in all treatment groups. While there was no significant difference in TAS levels compared to the negative control, TOS, GSH, and MDA levels were dose-dependently regulated. Treatment groups other than the high-dose group regulated the decrease in PCNA and the increase in caspase-3. CRYAA gene expression increased significantly only at the lowest dose. Conclusion: YM, which is becoming increasingly popular as a traditional tea, showed a therapeutic effect on hydrocortisone-induced cataracts in chicken embryos at relatively low doses.

Açıklama

Anahtar Kelimeler

Cataract, Chick embryo, CRYAA, Ilex paraguariensis, Yerba mate

Kaynak

Life

WoS Q Değeri

Q1

Scopus Q Değeri

Q1

Cilt

14

Sayı

8

Künye

Öncü, Ş., Kızılkaya, M. B., Bilir, A., Sarıtaş, A., Söylemez, E. S. A., Koca, H. B. ve diğerleri. (2024). Anti-Cataract effect of the traditional aqueouse extract of yerba mate (Ilex paraguariensis A. St.-Hil.): An in ovo perspective. Life, 14(8), 1-15.